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Asst. Prof. Yuki NAKAYA (Dr. Sc.)

Faculty of Engineering, Hokkaido University

Research Interest

Application of solid-phase fluorescence excitation emission matrix (SPF-EEM) spectroscopy to environmental samples

• Analysis of soil and humic substances (A-5, OP-1, OP-2, OP-4, PP-8)
• Establishment of IFE correction theory (A-10, A-15)
• Analysis of rare samples such as meteorites and polar deposits
• Fouling substances in membranes for water treatment
• Comparison with solid-state ¹³C-NMR spectroscopy

Estimation of the origin of rainwater derived infiltration and inflow (RDII) by focusing on “water quality”

• EEM spectroscopy and PARAFAC
• Bacterial community structure
• Inorganic ion
• Orbitrap-MS / ¹H-NMR spectroscopy

Diagnosis of water environment and water treatment process by image analysis

• Activated sludge floc (sedimentation, dewatering, bacterial adsorption capacity) (A-11, B-3, OP-3, PP-5)
• Quantification of filamentous bacteria
• Suspended solids (SS)
• Anaerobic digestion sludge (dewatering)
• Biofilm and periphyton
• Moving image analysis of activated sludge sedimentation and coagulation (OP-3, PP-6)

Analytical chemistry for sensor development (B-2)

1. Development and application of a colorimetric method for DNA and RNA using DNA-modified gold nanoparticles

• Method development and mechanism elucidation (A-9, A-12)
• Ammonia oxidizing bacteria (A-9, A-12)
• Enterohaemorrhagic Escherichia coli (O157) (A-9)
• Coronavirus
• Influenza virus

2. Simple, rapid and multi-sample quantification of bacteria by microplate reader (MPR) and specific enzyme substrate medium

• Escherichia coli (A-13)
• Total coliform
• Antibiotic-resistant enterococci and Pseudomonas aeruginosa

3. Development of DNA aptamers

• Arsenic (A-7)
• Legionella pneumophila

Elucidation of adsorption mechanism of activated sludge

• Kinetic analysis (PP-7)
• Adsorption isotherm
• Adsorption of Escherichia coli (A-16)
• Adsorption of suspended solids
• Simple analysis of surface physicochemical characteristics

Analysis of the water environment

• Diffusion of sewage and treated sewage into the environment
• EEM spectroscopy and PARAFAC
• Bacterial community structure
• Inorganic ion
• Orbitrap-MS / ¹H-NMR spectroscopy
• Tracing antibiotics and antibiotic-resistance genes
• Regrowth of Escherichia coli
• Classification of COD (natural or anthropogenic)

Wastewater surveillance focusing on pharmaceutical ingredients in wastewater

• Antipyretic analgesics
• Antibiotics
• Medicines for lifestyle-related diseases
• Supplements

Exploration of unknown bacteria and functions; Establishment of Culture Method

• Substrate depletion tracking (A-8)
• PET Manufacturing Wastewater Treatment (A-8, A-17)
•  Granule (A-14)

Formation and decomposition of humic substances in geochemical time scale

• Kinetics of the Maillard reaction (A-1, A-2, A-3, A-4, PP-1, PP-2, PP-3, PP-4)
•  Hydrothermal decomposition of humic substances (A-4)
• CO₂ production from humic substances (A-6)
• Organic / inorganic interaction kinetics (A-3, A-6, PP-4)

Publications

Books

• Nakaya, Y. and Nakashima, S. (2020)
  “5-3 Shionoyu hot spring”
  in: Hot springs in Japan, Supervised by The Japanese Society of Hot Spring Sciences, Asakura Publishing, p123. (in Japanese)

A. Original Papers

• A-1. Nakaya, Y. and Nakashima, S. (2016)
  In situ IR transmission spectroscopic observation and kinetic analyses of initial stage of the Maillard reaction as a simulated formation process of humic substances.
  Chemistry Letters, 45, 1204-1206, doi:10.1246/cl.160596
• A-2. Nakaya, Y., Nakashima, S. and Moriizumi, M. (2018)
  Non-destructive spectroscopic tracing of simulated formation processes of humic-like substances based on the Maillard reaction.
  Applied Spectroscopy,  72, 1189-1198, doi: 10.1177/0003702818775737
• A-3. Nakaya, Y., Okada, K., Ikuno, Y. and Nakashima, S. (2018)
  Spectroscopic study of effects of goethite surfaces on the simulated maillard reaction forming humic-like substances.
  e-Journal of Surface Science and Nanotechnology, 16, 411-418, doi: 10.1380/ejssnt.2018.411
• A-4. Nakaya, Y., Nakashima, S. and Otsuka, T. (2019)
  Evaluation of kinetic competition among formation and degradation processes of dissolved humic-like substances based on hydrothermal reactions measured by Ultraviolet–visible spectroscopy.
  Geochemical Journal, 53, 407-414, doi: 10.2343/geochemj.2.0575
• A-5. Nakaya, Y., Nakashima, S., Moriizumi, M., Oguchi, M., Kashiwagi, S. and Naka, N. (2020)
  Three dimensional excitation-emission matrix fluorescence spectroscopy of typical Japanese soil powders.
  Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 233, 118188,
  doi: 10.1016/j.saa.2020.118188
• A-6. Nakaya, Y., Nakashima, S. and Otsuka, T. (2021)
  Gas Cell Infrared and Attenuated Total Reflection Infrared Spectroscopic Studies for Organic-Inorganic Interactions in Adsorption of Fulvic Acid on the Goethite Surface Generating Carbon Dioxide.
  Applied spectroscopy, 75, 1114-1123,
  Online published, doi: 10.1177/0003702821991219
• A-7. Matsunaga, K., Nakaya, Y. and Satoh, H. (2022)
  Separate determination of arsenite and arsenate in groundwater samples by simple analytical methods.
  Water Supply, 22, 7635-7672,
  doi: 10.2166/ws.2022.331
• A-8. Kuroda, K., Narihiro, T., Nakaya, Y., Noguchi, T. Q. P., Maeda, R., Nobu, M. K., Ohnishi, Y., Kumaki, Y., Aizawa, T. and Satoh, H. (2022)
  Elucidation of the biodegradation pathways of bis(2-hydroxyethyl) terephthalate and dimethyl terephthalate under anaerobic conditions revealed by enrichment culture and microbiome analysis.
  Chemical Engineering Journal, 450, 137916.
  doi: 10.1016/j.cej.2022.137916
• A-9. Satoh, H., Nakajima, M., Hirano, R. and Nakaya, Y. (2022)
  Highly sensitive and homogeneous detection of unamplified RNA based on the light scattering properties of gold nanoparticle probes.
  Biosensors and Bioelectronics: X, 12, 100249.
  doi: 10.1016/j.biosx.2022.1
• A-10. Nakaya, Y., Tomita, A., Ochiai, K. and Yamamura, H. (2023)
  Quantification of organic fluorophores in absorbing media by solid-phase fluorescence excitation–emission matrix (SPF-EEM) spectroscopy of modeled mixtures containing bovine serum albumin (BSA) and colorants.
  Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 285, 121885.
  doi: 10.1016/j.saa.2022.12
• A-11. Nakaya, Y., Jia, J. and Satoh, H. (2023)
  Tracing morphological characteristics of activated sludge flocs by using a digital microscope and their effects on sludge dewatering and settling.
  Environmental Technology, online-published. doi: 10.1080/09593330.2023.2240026
• A-12. Nakajima, M., Hirano, R., Nakaya, Y. and Satoh, H. (2023)
  Analysis of Unamplified 16S rRNA of Ammonia-Oxidizing Bacteria in Activated Sludge by Spectrophotometry Using Gold Nanoprobes.
  ACS EST Water, 3, 3113-3120. doi: 10.1021/acsestwater.3c00265
• A-13. Shayan, M. N. M., Tanaka, Y., Hirano, R., Nakaya, Y. and Satoh, H. (2023)
  A simple and rapid method for detecting fecal pollution in urban rivers by measuring the intrinsic β-D-glucuronidase activity of Escherichia coli.
  Water Research, 246, 120689. doi: 10.1016/j.watres.2023.120689
• A-14. Oshiki, M., Saito, T., Nakaya, Y., Satoh, H. and Okabe, S. (2023)
  Growth of the Nitrosomonas europaea cells in the biofilm and planktonic growth mode: Responses of extracellular polymeric substances production and transcriptome.
  Journal of Bioscience and Bioengineering, 136, 430-437. doi: 10.1016/j.jbiosc.2023.10.002
• A-15. Nakaya, Y., Tomita, A. and Yamamura, H. (2024)
  Solid-phase fluorescence: Reproducibility and comparison with the solution states.
  Talanta, 270, 125566. doi: 10.1016/j.talanta.2023.125566
• A-16. Nakaya, Y., Nagahashi, N., Hirano, R., Ishizuka, Y. and Satoh, H. (2024)
  Physicochemical parameters affecting the adhesion of ciprofloxacin-resistant Escherichia coli to activated sludge.
  Water Science & Technology, 89, 2457-2467. doi: 10.2166/wst.2024.134
• A-17. Kuroda, K., Takai, M., Sekiguchi, T., Ikarashi, T., Kurashita, H., Nakajima, M, Nobu, M. K., Hatamoto, M., Yamaguchi, T.,  Nakaya, Y., Satoh, H., Yamauchi, M., Yamada, M. and  Narihiro, T. (2024)
  Development of an internal two-stage upflow anaerobic reactor integrating biostimulation strategies to enhance the degradation of aromatic compounds in wastewater from purified terephthalic acid and dimethyl terephthalate manufacturing processes.
  Water Research, 258, 121762. doi: 10.1016/j.watres.2024.121762

Presentation List

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